Utilization of renewable agricultural residues for the production of extracellular halostable cellulase from newly isolated halomonas sp. strain PS47

Abstract

A newly isolated biopolymer-degrading halophilic bacterium, Halomonas sp. strain PS47, yielded higher cellulase activity (0.0076 U/ml) in mineral salt medium (MM63). Activity increased to 0.029 U/ml when carboxymethyl cellulose (0.5 % w/v) was used as carbon source and further to 0.138 U/ml when a combination of yeast extract and peptone was used as nitrogen source. Enzyme secretion was maximal during late exponential and stationary phases (0.15 U/ml, 48 h). Among different agro-residues (1 % w/v), wheat bran gave the highest activity (0.12 U/ml) at pH 7.5, 30 �C and 6 % (w/v) NaCl. The cellulase exhibited higher activity at pH 7.1 and 50 �C. The enzyme exhibited activity over a wide range of NaCl concentrations (0-4 M). Optimum activity was at 0-1 M NaCl. At 4 M NaCl, activity was reduced to 65 % of the initial value. The present investigation thus contributes to the limited information available on halostable cellulases. � Springer-Verlag Berlin Heidelberg and the University of Milan 2012.